Patologia Generale/Immunologia

Dettagli

Chi siamo sitoweb

 

Responsabile scientifico:

Prof.ssa Teresa ZELANTE

Personale afferente:

  • Teresa ZELANTE   Questo indirizzo email è protetto dagli spambots. È necessario abilitare JavaScript per vederlo.
  • Gianluca VASCELLI   Questo indirizzo email è protetto dagli spambots. È necessario abilitare JavaScript per vederlo.
  • Undergraduated students doing final year project in faculty of medicine, lab.technician, PhD students
  • External collaborators from University of Brno (Czech republic), University of Manchester (UK)
 zelante 

Dove siamo

Edificio C piano 4, P.zza Lucio Severi, 1 Sant’Andrea delle Fratte 06129 Perugia, Italy

Cosa facciamo

Infiammazione e risposte del sistema immunitario (Teresa ZELANTE)
    My PhD was based on the study of the immunology of opportunistic diseases. In particular, in understanding the role of the Th17 cell response in early and late phases of infection. At the Singapore Immunology Network, I had my postdoc where I investigated the role of IL-2 in innate immune cells.
Alterazioni del metabolismo (Teresa ZELANTE)
    Directly under the Program Research ‘Giovani Ricercatori Rita Levi Montalcini’, I have found that microbes in the lung may activate a pathogenic response AhR-mediated. Therefore, in my research programs, I had the chance to develop expertise in Microbiology and Immunology. In the last years, I have focused on mouth/lung/gut microbiome studies by using as interface the 3D organoid model.
Malattie infettive (Teresa ZELANTE)
    We found that the Th17 pathway dampens tryptophan catabolism, and thus may favour pathology and accommodate the seemingly paradoxical association of chronic inflammation in fungal diseases. We also studied whether the cytokine IL-17A/F affected Candida gene transcription. Here, we found that chronic exposure to IL-17A/F affected Candida survival and germination rate, whereas short exposure induced the autophagic machinery response. We also found that a membrane protein bound the cytokine and was responsible for the intracellular response of Candida to IL-17A.
Interazione ospite/microbioma (Teresa ZELANTE)
    We are focused in understanding the oral microbiome in vivo systems of oral mucositis as well as in vitro systems by using oral tongue organoids. Our main aim is to provide novel therapies for oral mucositis in patients ndergoing radiotherapy
Scienze Omiche Integrate alla Medicina di Precisione (Teresa ZELANTE) sitoweb
    Studies focused on the microbe-host interaction based on more than 20 years of research experience in mechanisms of interaction between the host immune system and fungi as opportunistic pathogens. In particular, almost ten years ago, we have found that the mammalian enzyme IDO helps in regulating the homeostasis of the gut microbiome. Increased availability of tryptophan in vivo (simulated by using IDO-deficient mice) perturbs the system by favouring survival and proliferation of Lactobacilli, in particular L. reuteri, which is able to metabolize tryptophan and release secondary products that bind the AhR
Strumenti Innovativi e Nuove Tecnologie per la Tutela della Salute (Teresa ZELANTE) sitoweb
    Developing of organoids from healthy and cancer stem cells. The technology is extended on various tissues and biopsies and it has been founded recently by internal resources.

Tecnologie e Strumentazione

Pre-Clinical Models
    Model systems for cell biology (3D models starting from iPS cells or murine and human biopsy, tongue organoids, tumoroids), fungal infection models (gut, oral, vaginal and respiratory infections).
    Cell culture rooms, microscope, time lapse microscopy.

Pubblicazioni

Romani L., Fallarino F., De Luca A., Montagnoli C., D’Angelo C., Zelante T., Vacca C., Bistoni F., Fioretti M.C., Grohmann U., Segal B.H., Puccetti P. Defective tryptophan catabolism underlines inflammation in mouse chronic granulomatous disease. Nature. 451:211-5, 2008. doi: 10.1038/nature06471. PMID:18185592

Zelante T, Iannitti RG, Cunha C, De Luca A, Giovannini G, Pieraccini G, Zecchi R, D'Angelo C, Massi-Benedetti C, Fallarino F, Carvalho A, Puccetti P, Romani L. Tryptophan Catabolites from Microbiota Engage Aryl Hydrocarbon Receptor and Balance Mucosal Reactivity via Interleukin-22. Immunity. 2013 Aug 22;39(2):372-85. doi: 10.1016/j.immuni.2013.08.003.PMID:23973224

Bessede A, Gargaro M, Pallotta MT, Matino D, Servillo G, Brunacci C, Bicciato S, Mazza EM, Macchiarulo A, Vacca C, Iannitti R, Tissi L, Volpi C, Belladonna ML, Orabona C, Bianchi R, Lanz TV, Platten M, Della Fazia MA, Piobbico D, Zelante T, Funakoshi H, Nakamura T, Gilot D, Denison MS, Guillemin GJ, DuHadaway JB, Prendergast GC, Metz R, Geffard M, Boon L, Pirro M, Iorio A, Veyret B, Romani L, Grohmann U, Fallarino F, Puccetti P. Aryl hydrocarbon receptor control of a disease tolerance defence pathway. Nature. 2014 Jul 10;511(7508):184-90. doi: 10.1038/nature13323.PMID:24930766

Zelante T, Iannitti RG, Fallarino F, Gargaro M, De Luca A, Moretti S, Bartoli A, Romani L. Tryptophan Feeding of the IDO1-AhR Axis in Host-Microbial Symbiosis. Front Immunol. 2014 Dec 15;5:640. doi: 10.3389/fimmu.2014.00640. PMID:25566253

Armstrong-James D, Brown GD, Netea MG, Zelante T, Gresnigt MS, van de Veerdonk FL, Levitz SM. Immunotherapeutic approaches to treatment of fungal disease. Lancet Infect Dis 2017;17:e393

Host and Microbial Tryptophan Metabolic Profiling in Multiple Sclerosis. Gaetani L, Boscaro F, Pieraccini G, Calabresi P, Romani L, Di Filippo M, Zelante T. Front Immunol. 2020 Feb 18;11:157. doi: 10.3389/fimmu.2020.00157. eCollection 2020. PMID: 32132996

Using Lung Organoids to Investigate Epithelial Barrier Complexity and IL-17 Signaling During Respiratory Infection. Paolicelli G, Luca A, Jose SS, Antonini M, Teloni I, Fric J, Zelante T. Front Immunol. 2019 Feb 28;10:323.

The Use of Cre/loxP Inducible Mouse Models to Dissect the Specific Roles of Calcineurin Signaling in Myeloid Cells. Zelante T, Frič J. Methods Mol Biol. 2019;1929:223-231. doi: 10.1007/978-1-4939-9030-6_14.

Calcineurin inhibitors reduce NFAT-dependent expression of antifungal pentraxin-3 by human monocytes. Bendíčková K, Tidu F, De Zuani M, Kohoutková MH, Andrejčinová I, Pompeiano A, Bělášková S, Forte G, Zelante T, Frič J. J Leukoc Biol. 2020 Mar;107(3):497-508. doi: 10.1002/JLB.4VMA0318-138R

Aspergillus fumigatus tryptophan metabolic route differently affects host immunity. Zelante T, Choera T, Beauvais A, Fallarino F, Paolicelli G, Pieraccini G, Pieroni M, Galosi C, Beato C, De Luca A, Boscaro F, Romoli R, Liu X, Warris A, Verweij PE, Ballard E, Borghi M, Pariano M, Costantino G, Calvitti M, Vacca C, Oikonomou V, Gargaro M, Wong AYW, Boon L, den Hartog M, Spáčil Z, Puccetti P, Latgè JP, Keller NP, Romani L. Cell Rep. 2021 Jan 26;34(4):108673. doi: 10.1016/j.celrep.2020.108673

Zelante T, Paolicelli G, Fallarino F, Gargaro M, Vascelli G, De Zuani M, Fric J, Laznickova P, Kohoutkova MH, Macchiarulo A, Dolciami D, Pieraccini G, Gaetani L, Scalisi G, Trevisan C, Frossi B, Pucillo C, De Luca A, Nunzi E, Spaccapelo R, Pariano M, Borghi M, Boscaro F, Romoli R, Mancini A, Gentili L, Renga G, Costantini C, Puccetti M, Giovagnoli S, Ricci M, Antonini M, Calabresi P, Puccetti P, Di Filippo M, Romani L.
Sci Rep. 2024 Mar 20;14(1):6651. doi: 10.1038/s41598-024-57400-8.
PMID: 38509264

Activation of TLRs by Opportunistic Fungi in Lung Organoids.
Bosáková V, Frič J, Zelante T.Methods Mol Biol. 2023;2700:271-284. doi: 10.1007/978-1-0716-3366-3_16.
PMID: 37603187