| Responsabile scientifico:
Prof. Riccardo CALAFIORE Personale afferente:
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Dove siamo
Edificio D piano 0 P.zza Lucio Severi,1, Sant’Andrea delle Fratte 06132 Perugia, Italia.
Cosa facciamo
| Terapie innovative(Pia MONTANUCCI) |
| Studio del differenziamento verso il fenotipo insulare di cellule pluripotenti indotte per la terapia sostitutiva del Diabete Mellito di Tipo 1. |
| Terapie innovative (Giuseppe BASTA) |
| Produzione di microcapsule di alginato di sodio di grado clinico per trapianti contenenti cellule o tessuti. |
| Terapie innovative(Pia MONTANUCCI/Giuseppe BASTA) |
| Isolamento di cellule mesenchimali della matrice del cordone di Wharton per utilizzi di medicina rigenerativa in associazione all’utilizzo di stampanti 3D. |
Tecnologie e Strumentazione
| Genomics Metagenomics |
| Analyses of gene expression. |
| Proteomics and Metabolomics |
| Multyparametric flow cytometry. |
| Target Identification and Validation |
| Microscope analysis. |
| Pre-Clinical Models |
| Model system for cell biology (cell culture, 3D culture, organoids, induced pluripotent stem cells, multipotent stromal cells). Alginate purification, Microencapsulation. |
| Cell culture room. |
Pubblicazioni
R. Calafiore, G. Basta, G. Luca, A. Lemmi, M.P. Montanucci, G. Calabrese, L. Racanicchi, F. Mancuso, P. Brunetti. “Microencapsulated pancreatic islet allografts into nonimmunosuppressed patients with type 1 diabetes: first two cases.” Diabetes Care. 2006;29:137-138.
P. Montanucci, G. Basta, T. Pescara, I. Pennoni, F. Di Giovanni, R. Calafiore “New simple and rapid method for purification of mesenchymal stem cells from the human umbilical cord Wharton jelly.” Tissue Engineering Part A 2011;17:2651-61.
P. Montanucci, G. Basta, I. Pennoni, T. Pescara, P. Blasi, R. Calafiore “The functional performance of microencapsulated human pancreatic islet-derived precursor cells.” Biomaterials 2011;32:9254-9262
A. Alunno*, P. Montanucci*, O. Bistoni, G. Basta, S. Caterbi, T. Pescara, I. Pennoni, V. Bini, E. Bartoloni, R. Gerli, R. Calafiore. "In vitro immunomodulatory effects of microencapsulated umbilical cord Wharton jelly-derived mesenchymal stem cells in primary Sjögren's syndrome". Rheumatology (Oxford, England) 2015;54:163-168
P Montanucci, C di Pasquali, I Ferri, T Pescara, I Pennoni, P Siccu, A Sidoni, V Cervelli, G Basta, and R Calafiore, "Human Umbilical Cord Wharton Jelly-Derived Adult Mesenchymal Stem Cells, in Biohybrid Scaffolds, for Experimental Skin Regeneration," Stem Cells International, Volume 2017, 2017, Article number 1472642
F Morena, I* Armentano*, P Montanucci*, C Argentati, E Fortunati, S Montesano, I Bicchi, T Pescara, I Pennoni, S Mattioli, L Torre, L Latterini, C Emiliani, G Basta, R Calafiore, JM Kenny, S Martino. "Design of a nanocomposite substrate inducing adult stem cell assembly and progression toward an Epiblast-like or Primitive Endoderm-like phenotype via mechanotransduction." Biomaterials. 2017;144:211-229
Montanucci P, Pescara T, Alunno A, Bistoni O, Basta G, Calafiore R “Remission of hyperglycemia in spontaneously diabetic NOD mice upon transplant of microencapsulated human umbilical cord Wharton jelly‐derived mesenchymal stem cells (hUCMS)” Xenotransplantation. 2018;e12476
Montanucci P, Cari L, Basta G, Pescara T, Riccardi C, Nocentini G, Calafiore R. Engineered alginate microcapsules for molecular therapy trough biologic secreting cells Tissue Eng Part C Methods. 2019 Apr 5. doi: 10.1089/ten.TEC.2018.0329
Cari L*, Montanucci P*, Basta G, Petrillo MG, Ricci E, Pescara T, Greco A, Cipriani S, Shimizu J, Migliorati G, Nocentini G, Calafiore R, Riccardi C. “Microencapsulated G3C hybridoma cell graft delays the onset of spontaneous diabetes in NOD mice by an expansion of Gitr+ Treg cells”. Diabetes. 2020 May;69(5):965-980
Montanucci P, Pescara T, Greco A, Leonardi G, Marini L, Basta G, Calafiore R. “Co-microencapsulation of human umbilical cord-derived mesenchymal stem and pancreatic islet-derived insulin producing cells in experimental type 1 diabetes.” Diabetes Metab Res Rev. 2021;37:e337